Plasmid selection in Escherichia coli using an endogenous essential gene marker

Goh, S; Good, L

Abstract

Background: Antibiotic resistance genes are widely used for selection of recombinant bacteria, but their use risks contributing to the spread of antibiotic resistance. In particular, the practice is inappropriate for some intrinsically resistant bacteria and in vaccine production, and costly for industrial scale production. Non-antibiotic systems are available, but require mutant host strains, defined media or expensive reagents. An unexplored concept is over-expression of a host essential gene to enable selection in the presence of a chemical inhibitor of the gene product. To test this idea in E. coli, we used the growth essential target gene fabI as the plasmid-borne marker and the biocide triclosan as the selective agent.

Citation

Goh, S., & Good, L. Plasmid selection in Escherichia coli using an endogenous essential gene marker. BMC Biotechnology, 8(61), https://doi.org/10.1186/1472-6750-8-61

• Journal Article Type: Article
• Deposit Date: Nov 12, 2014
• Journal: BMC Biotechnology
• Electronic ISSN: 1472-6750
• Publisher: BioMed Central
• Volume: 8
• Issue: 61
• DOI: https://doi.org/10.1186/1472-6750-8-61
• Public URL: https://rvc-repository.worktribe.com/output/1429484