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All Outputs (140)

Attenuated but not virulent pseudorabies virus activates porcine bone marrow-derived dendritic cells (2025)
Journal Article
Ashley, E., Fuchs, W., Klupp, B., Werling, D., Graham, S., & Edwards, J. (2025). Attenuated but not virulent pseudorabies virus activates porcine bone marrow-derived dendritic cells. Veterinary Immunology and Immunopathology, 285, https://doi.org/10.1016/j.vetimm.2025.110960

Pseudorabies viruses (PrV), the causative agent of Aujeszky's disease, continues to cause economic losses to pig producers across Southeast Asia. PrV is controlled by vaccination with live attenuated vaccines, such as the Bartha K61 strain, which has... Read More about Attenuated but not virulent pseudorabies virus activates porcine bone marrow-derived dendritic cells.

Identification of DNA methylation markers for age and Bovine Respiratory Disease in dairy cattle: A pilot study based on Reduced Representation Bisulfite Sequencing (2024)
Journal Article
Attree, E., Griffiths, B., Panchal, K., Xia, D., Werling, D., Banos, G., Oikonomou, G., & Psifidi, A. (2025). Identification of DNA methylation markers for age and Bovine Respiratory Disease in dairy cattle: A pilot study based on Reduced Representation Bisulfite Sequencing. Communications Biology, 7(1), https://doi.org/10.1038/s42003-024-06925-9

Multi-omics analysis reveals regime shifts in the gastrointestinal ecosystem in chickens following anticoccidial vaccination and Eimeria tenella challenge (2024)
Journal Article
Liu, P.-Y., Liaw, J., Soutter, F., Ortiz, J. J., Tomley, F. M., Werling, D., Gundogdu, O., Blake, D. P., & Xia, D. (in press). Multi-omics analysis reveals regime shifts in the gastrointestinal ecosystem in chickens following anticoccidial vaccination and Eimeria tenella challenge. mSystems, https://doi.org/10.1128/msystems.00947-24

Characterisation of Canine Intestinal MicroRNA Expression in Inflammatory Bowel Disease and T-Cell Lymphoma (2023)
Journal Article
Williams, J., Werling, D., Davison, L., Hiron, T., & Xia, D. (in press). Characterisation of Canine Intestinal MicroRNA Expression in Inflammatory Bowel Disease and T-Cell Lymphoma. Journal of Comparative Pathology, https://doi.org/10.1016/j.jcpa.2023.03.186

Differentiating between canine inflammatory bowel disease and intestinal T-cell lymphoma via histopathological examination of endoscopically-derived intestinal biopsies can be challenging and involves an invasive procedure requiring specialised equip... Read More about Characterisation of Canine Intestinal MicroRNA Expression in Inflammatory Bowel Disease and T-Cell Lymphoma.

Characterisation of the bovine C-type lectin receptor Mincle and potential evidence for an endogenous ligand (2023)
Journal Article
Holder, A., Kolakowski, J., Rosentreter, C., Knuepfer, E., Jegouzo, S., Rosenwasser, O., Harris, H., Baumgaertel, L., Gibson, A., & Werling, D. (2023). Characterisation of the bovine C-type lectin receptor Mincle and potential evidence for an endogenous ligand. Frontiers in Immunology, 14, https://doi.org/10.3389/fimmu.2023.1189587

Innate immune receptors that form complexes with secondary receptors, activating multiple signalling pathways, modulate cellular activation and play essential roles in regulating homeostasis and immunity. We have previously identified a variety of bo... Read More about Characterisation of the bovine C-type lectin receptor Mincle and potential evidence for an endogenous ligand.

East Coast fever, a neglected tropical disease with an outdated vaccine approach? (2022)
Journal Article
Kolakowski, J., Connelley, T., Lukacik, P., Pfuhl, M., & Werling, D. (2022). East Coast fever, a neglected tropical disease with an outdated vaccine approach?. Trends in Parasitology, 38(11), 930-932. https://doi.org/10.1016/j.pt.2022.08.006

Since its discovery, bovine theileriosis has caused major socioeconomic losses in sub-Saharan Africa. Acaricide resistance of the intermediate host, paucity of therapeutics, and lack of sufficiently cross-protective vaccines increase the risk of para... Read More about East Coast fever, a neglected tropical disease with an outdated vaccine approach?.

Transcriptomic analysis of circulating leukocytes obtained during the recovery from clinical mastitis caused by Escherichia coli in Holstein dairy cows (2022)
Journal Article
Cheng, Z., Palma-Vera, S., Buggiotti, L., Salavati, M., Becker, F., Werling, D., Wathes, D. C., & consortium, G. (in press). Transcriptomic analysis of circulating leukocytes obtained during the recovery from clinical mastitis caused by Escherichia coli in Holstein dairy cows. Animals, 12(16), 2146. https://doi.org/10.3390/ani12162146

The risk and severity of clinical infection with Escherichia coli as a causative pathogen for bovine mastitis is influenced by the hosts’ phenotypic and genotypic variables. We used RNA-Seq analysis of circulating leukocytes to investigate global tra... Read More about Transcriptomic analysis of circulating leukocytes obtained during the recovery from clinical mastitis caused by Escherichia coli in Holstein dairy cows.

Defining the genes required for survival of Mycobacterium bovis in the bovine host offers novel insights into the genetic basis of survival of pathogenic mycobacteria (2022)
Journal Article
Gibson, A. J., Stiens, J., Passmore, I. J., Faulkner, V., Miculob, J., Willcocks, S., Coad, M., Berg, S., Werling, D., Wren, B. W., Nobeli, I., Villarreal-Ramos, B., & Kendall, S. L. (2022). Defining the genes required for survival of Mycobacterium bovis in the bovine host offers novel insights into the genetic basis of survival of pathogenic mycobacteria. mBio, https://doi.org/10.1128/mbio.00672-22

Tuberculosis has severe impacts in both humans and animals. Understanding the genetic basis of survival of both Mycobacterium tuberculosis, the human adapted species, and Mycobacterium bovis, the animal adapted species is crucial to deciphering the b... Read More about Defining the genes required for survival of Mycobacterium bovis in the bovine host offers novel insights into the genetic basis of survival of pathogenic mycobacteria.

Development of a yeast-based vaccine for Theileria parva infection in cattle (2022)
Journal Article
Werling, D., Goh, S., Kolakowski, J., Holder, A., Pfuhl, M., Ngugi, D., Ballingall, K., Tombacz, K., & Werling, D. (2022). Development of a yeast-based vaccine for Theileria parva infection in cattle. npj Vaccines, https://doi.org/10.3389/fimmu.2021.674484

East Coast Fever (ECF), caused by the tick-borne apicomplexan parasite Theileria parva, remains one of the most important livestock diseases in sub-Saharan Africa with more than 1 million cattle dying from infection every year. Disease prevention rel... Read More about Development of a yeast-based vaccine for Theileria parva infection in cattle.

A novel whole yeast-based subunit oral vaccine against Eimeria tenella in chickens (2022)
Journal Article
Soutter, F., Werling, D., Nolan, M., Küster, T., Attree, E., Marugán-Hernández, V., Kim, S., Tomley, F. M., & Blake, D. (2022). A novel whole yeast-based subunit oral vaccine against Eimeria tenella in chickens. Frontiers in Immunology, 13, Article 809711. https://doi.org/10.3389/fimmu.2022.809711

Cheap, easy-to-produce oral vaccines are needed for control of coccidiosis in chickens to reduce the impact of this disease on welfare and economic performance. Saccharomyces cerevisiae yeast expressing three Eimeria tenella antigens were developed a... Read More about A novel whole yeast-based subunit oral vaccine against Eimeria tenella in chickens.

Human-relevant near-organ neuromodulation of the immune via the nerve (2021)
Journal Article
Donega, M., Fjordbakk, C., Kirk, J., Sokal, D., Gupta, I., Hunsberger, G., Crawford, A., Cook, S., Viscasillas, J., Stathopoulou, T., Miranda, J., Dopson, W., Goodwin, D., Rowles, A., McGill, P., McSloy, A., Werling, D., Witherington, J., Chew, D., & Perkins, J. (2021). Human-relevant near-organ neuromodulation of the immune via the nerve. Proceedings of the National Academy of Sciences, 118(20), https://doi.org/10.1073/pnas.2025428118

Neuromodulation of immune function by stimulating the autonomic connections to the spleen has been demonstrated in rodent models. Consequently, neuroimmune modulation has been proposed as a new therapeutic strategy for the treatment of inflammatory c... Read More about Human-relevant near-organ neuromodulation of the immune via the nerve.

Splenic Nerve Neuromodulation Reduces Inflammation and Promotes Resolution in Chronically Implanted Pigs (2021)
Journal Article
Sokal, D., McSloy, A., Donega, M., Kirk, J., Colas, R., Dolezalova, N., Gomez, E., Gupta, I., Fjordbakk, C., Ouchouche, S., Matteucci, P., Schlegel, K., Bashirullah, R., Werling, D., Harman, K., Rowles, A., Yazicioglu, R., Dalli, J., Chew, D., & Perkins, J. (2021). Splenic Nerve Neuromodulation Reduces Inflammation and Promotes Resolution in Chronically Implanted Pigs. Frontiers in Immunology, 12, https://doi.org/10.3389/fimmu.2021.649786

Neuromodulation of the immune system has been proposed as a novel therapeutic strategy for the treatment of inflammatory conditions. We recently demonstrated that stimulation of near-organ autonomic nerves to the spleen can be harnessed to modulate t... Read More about Splenic Nerve Neuromodulation Reduces Inflammation and Promotes Resolution in Chronically Implanted Pigs.

Development of a Potential Yeast-Based Vaccine Platform for Theileria parva Infection in Cattle (2021)
Journal Article
Goh, S., Kolakowski, J., Holder, A., Pfuhl, M., Ngugi, D., Ballingall, K., Tombacz, K., & Werling, D. (2021). Development of a Potential Yeast-Based Vaccine Platform for Theileria parva Infection in Cattle. Frontiers in Immunology, 12, https://doi.org/10.3389/fimmu.2021.674484

East Coast Fever (ECF), caused by the tick-borne apicomplexan parasite Theileria parva, remains one of the most important livestock diseases in sub-Saharan Africa with more than 1 million cattle dying from infection every year. Disease prevention rel... Read More about Development of a Potential Yeast-Based Vaccine Platform for Theileria parva Infection in Cattle.

Bovine Neutrophils Release Extracellular Traps and Cooperate With Macrophages in Mycobacterium avium subsp. paratuberculosis clearance In Vitro (2021)
Journal Article
Ladero-Aunon, I., Molina, E., Holder, A., Kolakowski, J., Harris, H., Urkitza, A., Anguita, J., Werling, D., & Elguezabal, N. (2021). Bovine Neutrophils Release Extracellular Traps and Cooperate With Macrophages in Mycobacterium avium subsp. paratuberculosis clearance In Vitro. Frontiers in Immunology, 12, https://doi.org/10.3389/fimmu.2021.645304

Mycobacterium avium subsp. paratuberculosis (Map) is the underlying pathogen causing bovine paratuberculosis (PTB), an enteric granulomatous disease that mainly affects ruminants and for which an effective treatment is needed. Macrophages are the pri... Read More about Bovine Neutrophils Release Extracellular Traps and Cooperate With Macrophages in Mycobacterium avium subsp. paratuberculosis clearance In Vitro.

Macrophage-specific responses to human- and animal-adapted tubercle bacilli reveal pathogen and host factors driving multinucleated cell formation (2021)
Journal Article
Queval, C., Fearns, A., Botella, L., Smyth, A., Schnettger, L., Mitermite, M., Wooff, E., Villarreal-Ramos, B., Garcia-Jimenez, W., Heunis, T., Trost, M., Werling, D., Salguero, F., Gordon, S., & Gutierrez, M. (2021). Macrophage-specific responses to human- and animal-adapted tubercle bacilli reveal pathogen and host factors driving multinucleated cell formation. PLoS Pathogens, 17(3), https://doi.org/10.1371/journal.ppat.1009410

The Mycobacterium tuberculosis complex (MTBC) is a group of related pathogens that cause tuberculosis (TB) in mammals. MTBC species are distinguished by their ability to sustain in distinct host populations. While Mycobacterium bovis (Mbv) sustains t... Read More about Macrophage-specific responses to human- and animal-adapted tubercle bacilli reveal pathogen and host factors driving multinucleated cell formation.

Activation of Dendritic Cells in Tonsils Is Associated with CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus (2021)
Journal Article
Soldevila, F., Edwards, J., Graham, S., Crooke, H., Werling, D., & Steinbach, F. (2021). Activation of Dendritic Cells in Tonsils Is Associated with CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus. International Journal of Molecular Sciences, 22(16), https://doi.org/10.3390/ijms22168795

Classical swine fever (CSF) is a highly contagious disease caused by the classical swine fever virus (CSFV). The live attenuated C-strain vaccine is highly efficacious, initiating protection within several days of delivery. The vaccine strain is dete... Read More about Activation of Dendritic Cells in Tonsils Is Associated with CD8 T Cell Responses following Vaccination with Live Attenuated Classical Swine Fever Virus.

Probing Differences in Gene Essentiality Between the Human and Animal Adapted Lineages of the Mycobacterium tuberculosis Complex Using TnSeq (2021)
Journal Article
Gibson, A., Passmore, I., Faulkner, V., Xia, D., Nobeli, I., Stiens, J., Willcocks, S., Clark, T., Sobkowiak, B., Werling, D., Villarreal-Ramos, B., Wren, B., & Kendall, S. (2021). Probing Differences in Gene Essentiality Between the Human and Animal Adapted Lineages of the Mycobacterium tuberculosis Complex Using TnSeq. Frontiers in Veterinary Science, 8, https://doi.org/10.3389/fvets.2021.760717

Members of the Mycobacterium tuberculosis complex (MTBC) show distinct host adaptations, preferences and phenotypes despite being >99% identical at the nucleic acid level. Previous studies have explored gene expression changes between the members, ho... Read More about Probing Differences in Gene Essentiality Between the Human and Animal Adapted Lineages of the Mycobacterium tuberculosis Complex Using TnSeq.

Probing differences in gene essentiality between the human and animal adapted (2021)
Journal Article
Gibson, A. J., Passmore, I. J., Faulkner, V., Xia, D., Nobeli, I., Stiens, J., Clarke, T. G., Sobkowiak, B., Willcocks, S., Werling, D., Villarreal-Ramos, B., Wren, B. W., & Kendall, S. L. (2021). Probing differences in gene essentiality between the human and animal adapted. Frontiers in Veterinary Science,

Members of the Mycobacterium tuberculosis complex (MTBC) show distinct host adaptations, preferences and phenotypes despite being >99% identical at the nucleic acid level. Previous studies have explored gene expression changes between the members, ho... Read More about Probing differences in gene essentiality between the human and animal adapted.