Skip to main content

Research Repository

Advanced Search

All Outputs (93)

Equine Embryonic Stem Cell-Derived Tenocytes are Insensitive to a Combination of Inflammatory Cytokines and Have Distinct Molecular Responses Compared to Primary Tenocytes (2024)
Journal Article
Smith, E. J., Beaumont, R. E., Dudhia, J., & Guest, D. J. (in press). Equine Embryonic Stem Cell-Derived Tenocytes are Insensitive to a Combination of Inflammatory Cytokines and Have Distinct Molecular Responses Compared to Primary Tenocytes. Stem Cell Reviews and Reports, https://doi.org/10.1007/s12015-024-10693-8

Tissue fibrosis following tendon injury is a major clinical problem due to the increased risk of re-injury and limited treatment options; however, its mechanism remains unclear. Evidence suggests that insufficient resolution of inflammation contribut... Read More about Equine Embryonic Stem Cell-Derived Tenocytes are Insensitive to a Combination of Inflammatory Cytokines and Have Distinct Molecular Responses Compared to Primary Tenocytes.

Technical note: Cartilage imaging with sub-cellular resolution using a laboratory-based phase-contrast x-ray microscope (2023)
Journal Article
Esposito, M., Astolfo, A., Cipiccia, S., Jones, C., Savvidis, S., Ferrara, J., Endrizzi, M., Dudhia, J., & Olivo, A. (2023). Technical note: Cartilage imaging with sub-cellular resolution using a laboratory-based phase-contrast x-ray microscope. Medical Physics, https://doi.org/10.1002/mp.16599

BackgroundMicroscopic imaging of cartilage is a key tool for the study and development of treatments for osteoarthritis. When cellular and sub-cellular resolution is required, histology remains the gold standard approach, albeit limited by the lack o... Read More about Technical note: Cartilage imaging with sub-cellular resolution using a laboratory-based phase-contrast x-ray microscope.

Exploration of Mediators Associated with Myocardial Remodelling in Feline Hypertrophic Cardiomyopathy (2023)
Journal Article
Cheng, W.-C., Lawson, C., Liu, H.-H., Wilkie, L., Dobromylskyj, M., Fuentes, V. L., Dudhia, J., & Connolly, D. J. (2023). Exploration of Mediators Associated with Myocardial Remodelling in Feline Hypertrophic Cardiomyopathy. Animals, 13(13), 2112. https://doi.org/10.3390/ani13132112

Simple Summary: The hallmark changes in hypertrophic cardiomyopathy (HCM), a naturally occurring heart disease in both humans and cats, are left ventricular hypertrophy (LVH) and myocardial fibrosis. Key myocardial proteins, lumican, lysyl oxidase (L... Read More about Exploration of Mediators Associated with Myocardial Remodelling in Feline Hypertrophic Cardiomyopathy.

Transdermal drug delivery in horses: An in vitro comparison of skin structure and permeation of two model drugs at various anatomical sites (2023)
Journal Article
Bizley, S., Dudhia, J., Smith, R., & Williams, A. (2023). Transdermal drug delivery in horses: An in vitro comparison of skin structure and permeation of two model drugs at various anatomical sites. Veterinary Dermatology, 34(3), 235-245. https://doi.org/10.1111/vde.13162

Background: Oral and parenteral drug delivery in horses can be difficult. Equine--specific transdermal drug formulations offer improved ease of treatment; development of such formulations requires a deeper understanding of the structural and chemical... Read More about Transdermal drug delivery in horses: An in vitro comparison of skin structure and permeation of two model drugs at various anatomical sites.

Tumour necrosis factor alpha, Interleukin 1 beta and Interferon gamma have detrimental effects on equine tenocytes that cannot be rescued by IL-1RA or mesenchymal stromal cell-derived factors (2022)
Journal Article
Guest, D., Smith, E., Beaumont, R., McClellan, A., Sze, C., Palomino Lago, E., Hazelgrove, L., Dudhia, J., & Smith, R. (in press). Tumour necrosis factor alpha, Interleukin 1 beta and Interferon gamma have detrimental effects on equine tenocytes that cannot be rescued by IL-1RA or mesenchymal stromal cell-derived factors. Cell and Tissue Research, https://doi.org/10.1007/s00441-022-03726-6

Tendon injuries occur commonly in both human and equine athletes, and poor tendon regeneration leads to functionally
deficient scar tissue and an increased frequency of re-injury. Despite evidence suggesting inadequate resolution of inflammation
le... Read More about Tumour necrosis factor alpha, Interleukin 1 beta and Interferon gamma have detrimental effects on equine tenocytes that cannot be rescued by IL-1RA or mesenchymal stromal cell-derived factors.

Position Statement: Minimal criteria for reporting veterinary and animal medicine research for mesenchymal stromal/stem cells in orthopaedic applications. (2022)
Journal Article
Guest, D. J., Dudhia, J., Smith, R. K., Roberts, S., Conzemius, M., Innes, J. F., Fortier, L., & Meeson, R. L. (2022). Position Statement: Minimal criteria for reporting veterinary and animal medicine research for mesenchymal stromal/stem cells in orthopaedic applications. Frontiers in Veterinary Science, https://doi.org/10.3389/fvets.2022.817041

Mesenchymal stromal/stem cells (MSCs) are being used in an increasingly broad range of clinical applications in horses, dogs and cats. In this position statement, we aim to provide guidelines to improve reporting research involving the use of MSCs in... Read More about Position Statement: Minimal criteria for reporting veterinary and animal medicine research for mesenchymal stromal/stem cells in orthopaedic applications..

CD146 Delineates an Interfascicular Cell Sub-Population in Tendon That Is Recruited during Injury through Its Ligand Laminin-α4 (2021)
Journal Article
Marr, N., Meeson, R., Kelly, E., Fang, Y., Peffers, M., Pitsillides, A., Dudhia, J., & Thorpe, C. (2021). CD146 Delineates an Interfascicular Cell Sub-Population in Tendon That Is Recruited during Injury through Its Ligand Laminin-α4. International Journal of Molecular Sciences, https://doi.org/10.3390/ijms22189729

The interfascicular matrix (IFM) binds tendon fascicles and contains a population of morphologically distinct cells; however the role of IFM-localised cell populations in tendon repair remains to be determined. The basement membrane protein laminin-α... Read More about CD146 Delineates an Interfascicular Cell Sub-Population in Tendon That Is Recruited during Injury through Its Ligand Laminin-α4.

Intra-operative Raman spectroscopy and ex vivo Raman mapping for assessment of cartilage degradation (2021)
Journal Article
Gaifulina, R., Nunn, A. D. G., Draper, E. R. C., Strachan, R. K., Blake, N., Firth, S., Thomas, G. M. H., Mcmillan, P. F., Dudhia, J., & 6ap, U. K. (2021). Intra-operative Raman spectroscopy and ex vivo Raman mapping for assessment of cartilage degradation. Applied Spectroscopy, https://doi.org/10.1016/j.clispe.2021.100012

The development of a label-free, non-destructive and safe analytical method such as Raman spectroscopy for assessing cartilage degradation is highly desirable. Compared to non-optical imaging modalities, Raman mapping offers a more sensitive means of... Read More about Intra-operative Raman spectroscopy and ex vivo Raman mapping for assessment of cartilage degradation.

Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do (2020)
Journal Article
Ribitsch, I., Baptista, P. M., Lange-Consiglio, A., Melotti, L., Patruno, M., Jenner, F., Schnabl-Feichter, E., Dutton, L. C., Connolly, D. J., van Steenbeek, F. G., Dudhia, J., & Penning, L. C. (2020). Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do. Frontiers in Bioengineering and Biotechnology, 8,

Rapid developments in Regenerative Medicine and Tissue Engineering has witnessed an increasing drive toward clinical translation of breakthrough technologies. However, the progression of promising preclinical data to achieve successful clinical marke... Read More about Large Animal Models in Regenerative Medicine and Tissue Engineering: To Do or Not to Do.

Heterogeneity of proteome dynamics between connective tissue phases of adult tendon (2020)
Journal Article
Choi, H., Simpson, D., Wang, D., Prescott, M., Pitsillides, A. A., Dudhia, J., Clegg, P. D., Ping, P., & Thorpe, C. T. (2020). Heterogeneity of proteome dynamics between connective tissue phases of adult tendon. eLife, 9, https://doi.org/10.7554/elife.55262

Maintenance of connective tissue integrity is fundamental to sustain function, requiring protein turnover to repair damaged tissue. However, connective tissue proteome dynamics remain largely undefined, as do differences in turnover rates of individu... Read More about Heterogeneity of proteome dynamics between connective tissue phases of adult tendon.

Development of a Cartilage Oligomeric Matrix Protein Neo-Epitope Assay for the Detection of Intra-Thecal Tendon Disease (2020)
Journal Article
Smith, R. K. W., Önnerfjord, P., Holmgren, K., di Grado, S., & Dudhia, J. (2020). Development of a Cartilage Oligomeric Matrix Protein Neo-Epitope Assay for the Detection of Intra-Thecal Tendon Disease. International Journal of Molecular Sciences, 21(6), 2155. https://doi.org/10.3390/ijms21062155

The diagnosis of tendon injury relies on clinical signs and diagnostic imaging but imaging is subjective and does not always correlate with clinical signs. A molecular marker would potentially offer a sensitive and specific diagnostic tool that could... Read More about Development of a Cartilage Oligomeric Matrix Protein Neo-Epitope Assay for the Detection of Intra-Thecal Tendon Disease.

Histopathological and immunohistochemical evaluation of cellular response to a woven and electrospun polydioxanone (PDO) and polycaprolactone (PCL) patch for tendon repair (2020)
Journal Article
Rashid, M., Dudhia, J., Dakin, S. G., Snelling, S. J. B., De Godoy, R., Mouthuy, P.-A., Smith, R. K. W., Morrey, M., & Carr, A. J. (2020). Histopathological and immunohistochemical evaluation of cellular response to a woven and electrospun polydioxanone (PDO) and polycaprolactone (PCL) patch for tendon repair. Scientific Reports, 10(1), https://doi.org/10.1038/s41598-020-61725-5

We investigated endogenous tissue response to a woven and electrospun polydioxanone (PDO) and polycaprolactone (PCL) patch intended for tendon repair. A sheep tendon injury model characterised by a natural history of consistent failure of healing was... Read More about Histopathological and immunohistochemical evaluation of cellular response to a woven and electrospun polydioxanone (PDO) and polycaprolactone (PCL) patch for tendon repair.

Inducing pluripotency in the domestic cat (Felis Catus) (2019)
Journal Article
Dutton, L. C., Dudhia, J., Guest, D. J., & Connolly, D. J. (2019). Inducing pluripotency in the domestic cat (Felis Catus). Stem Cells and Development, 28(19), 1299-1309. https://doi.org/10.1089/scd.2019.0142

Domestic cats suffer from a range of inherited genetic diseases, many of which display similarities with equivalent human conditions. Developing cellular models for these inherited diseases would enable drug discovery, benefiting feline health and we... Read More about Inducing pluripotency in the domestic cat (Felis Catus).

Evaluation of the Effects of Synovial Multipotent Cells on Deep Digital Flexor Tendon Repair in a Large Animal Model of Intra-Synovial Tendinopathy (2019)
Journal Article
Khan, M. R., Smith, R. K., David, F. H., Lam, R., Hughes, G., DeGodoy, R., Carr, A. J., Goodship, A. E., & Dudhia, J. (2019). Evaluation of the Effects of Synovial Multipotent Cells on Deep Digital Flexor Tendon Repair in a Large Animal Model of Intra-Synovial Tendinopathy. Journal of Orthopaedic Research, 38(1), 128-138. https://doi.org/10.1002/jor.24423

ntra‐synovial tendon injuries are a common orthopedic problem with limited treatment options. The synovium is a specialized connective tissue forming the inner encapsulating lining of diarthrodial joints and intra‐synovial tendons. It contains multip... Read More about Evaluation of the Effects of Synovial Multipotent Cells on Deep Digital Flexor Tendon Repair in a Large Animal Model of Intra-Synovial Tendinopathy.

Bone marrow mesenchymal stem cells do not enhance intra-synovial tendon healing despite engraftment and homing to niches within the synovium (2018)
Journal Article
Khan, M. R., Dudhia, J., David, F. H., De Godoy, R., Mehra, V., Hughes, G., Dakin, S. G., Carr, A. J., Goodship, A. E., & Smith, R. K. W. (2018). Bone marrow mesenchymal stem cells do not enhance intra-synovial tendon healing despite engraftment and homing to niches within the synovium. https://doi.org/10.1186/s13287-018-0900-7

Intra-synovial tendon injuries display poor healing, which often results in reduced functionality and pain. A lack of effective therapeutic options has led to experimental approaches to augment natural tendon repair with autologous mesenchymal stem c... Read More about Bone marrow mesenchymal stem cells do not enhance intra-synovial tendon healing despite engraftment and homing to niches within the synovium.