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Outputs (168)

Heterogeneity of proteome dynamics between connective tissue phases of adult tendon (2020)
Journal Article
Choi, H., Simpson, D., Wang, D., Prescott, M., Pitsillides, A. A., Dudhia, J., Clegg, P. D., Ping, P., & Thorpe, C. T. (2020). Heterogeneity of proteome dynamics between connective tissue phases of adult tendon. eLife, 9, https://doi.org/10.7554/elife.55262

Maintenance of connective tissue integrity is fundamental to sustain function, requiring protein turnover to repair damaged tissue. However, connective tissue proteome dynamics remain largely undefined, as do differences in turnover rates of individu... Read More about Heterogeneity of proteome dynamics between connective tissue phases of adult tendon.

Applied mechanical loading to mouse hindlimb acutely increases skeletal perfusion and chronically enhanced vascular porosity (2020)
Journal Article
Gohin, S., Javaheri, B., Hopkinson, M., Pitsillides, A. A., Arnett, T. R., & Chenu, C. (2020). Applied mechanical loading to mouse hindlimb acutely increases skeletal perfusion and chronically enhanced vascular porosity. Journal of Applied Physiology,

Blood supply is essential for osteogenesis, yet its relationship to load-related increases in bone mass is poorly defined. Herein, we aim to investigate the link between load-induced osteogenesis and the blood supply (bone perfusion and vascular poro... Read More about Applied mechanical loading to mouse hindlimb acutely increases skeletal perfusion and chronically enhanced vascular porosity.

Lasting organ-level bone mechanoadaptation is unrelated to local strain (2020)
Journal Article
Javaheri, B., Razi, H., Gohin, S., Wylie, S., Chang, Y. M., Salmon, P., Lee, P. D., & Pitsillides, A. A. (2020). Lasting organ-level bone mechanoadaptation is unrelated to local strain. Science Advances, 6(10), https://doi.org/10.1126/sciadv.aax8301

Bones adapt to mechanical forces according to strict principles predicting straight shape. Most bones are, however, paradoxically curved. To solve this paradox, we used computed tomography–based, four-dimensional imaging methods and computational ana... Read More about Lasting organ-level bone mechanoadaptation is unrelated to local strain.

Conditional deletion of E11/Podoplanin in bone protects against ovariectomy-induced increases in osteoclast formation and activity (2020)
Journal Article
Staines, K., Hopkinson, M., Dillon, S., Stephen, L., Fleming, R., Sophocleous, A., Buttle, D., Pitsillides, A., & Farquharson, C. (2020). Conditional deletion of E11/Podoplanin in bone protects against ovariectomy-induced increases in osteoclast formation and activity. Bioscience Reports, 40, https://doi.org/10.1042/BSR20190329

E11/Podoplanin (Pdpn) is implicated in early osteocytogenesis and the formation of osteocyte dendrites. This dendritic network is critical for bone modelling/remodelling, through the production of receptor activator of nuclear factor κ B (RANK)-ligan... Read More about Conditional deletion of E11/Podoplanin in bone protects against ovariectomy-induced increases in osteoclast formation and activity.

Aging and Mechanoadaptive Responsiveness of Bone (2019)
Journal Article
Javaheri, B., & Pitsillides, A. A. (2019). Aging and Mechanoadaptive Responsiveness of Bone. Current Osteoporosis Reports, 17(6), 560-569. https://doi.org/10.1007/s11914-019-00553-7

Osteoporosis is an age-related disorder characterized by bone loss and increased fracture susceptibility. Whether this is due to reduced loading in less active elderly individuals or inherent modifications in bone cells is uncertain. We suppose that... Read More about Aging and Mechanoadaptive Responsiveness of Bone.

Raman spectroscopy links differentiating osteoblast matrix signatures to pro-angiogenic potential (2019)
Journal Article
Sharma, A., Goring, A., Staines, K. A., Emery, R., Pitsillides, A. A., Oreffo, R. O. C., Mahajan, S., & Clarkin, C. E. (2019). Raman spectroscopy links differentiating osteoblast matrix signatures to pro-angiogenic potential. Matrix Biology, 5, https://doi.org/10.1016/j.mbplus.2019.100018

Mineralization of bone is achieved by the sequential maturation of the immature amorphous calcium phase to mature hydroxyapatite and is central to the process of bone development and repair. To study normal and dysregulated mineralization in vitro, s... Read More about Raman spectroscopy links differentiating osteoblast matrix signatures to pro-angiogenic potential.

Propagation phase-contrast micro-computed tomography allows laboratory-based three-dimensional imaging of articular cartilage down to the cellular level (2019)
Journal Article
Clark, J. N., Garbout, A., Ferreira, S. A., Javaheri, B., Pitsillides, A. A., Rankin, S. M., Jeffers, J. R. T., & Hansen, U. (2019). Propagation phase-contrast micro-computed tomography allows laboratory-based three-dimensional imaging of articular cartilage down to the cellular level. Osteoarthritis and Cartilage, 28, 102-111. https://doi.org/10.1016/j.joca.2019.10.007

High-resolution non-invasive three-dimensional (3D) imaging of chondrocytes in articular cartilage remains elusive. The aim of this study was to explore whether laboratory micro-computed tomography (micro-CT) permits imaging cells within articular ca... Read More about Propagation phase-contrast micro-computed tomography allows laboratory-based three-dimensional imaging of articular cartilage down to the cellular level.

Dchs1-Fat4 regulation of osteogenic differentiation in mouse (2019)
Journal Article
Crespo-Enriquez, I., Hodgson, T., Zakaria, S., Cadoni, E., Shah, M., Allen, S., Al-Krishali, A., Mao, Y., Yiu, A., Villagomez-Olea, G., Petzold, J., Pitsillides, A. A., Irvine, K., & Francis-West, P. (2019). Dchs1-Fat4 regulation of osteogenic differentiation in mouse. Development, 146, https://doi.org/10.1242/dev.176776

In human, mutations of the protocadherins FAT4 and DCHS1 result in Van Maldergem syndrome, which is characterised, in part, by craniofacial abnormalities. Here, we analyse the role of Dchs1-Fat4 signalling during osteoblast differentiation in mouse.... Read More about Dchs1-Fat4 regulation of osteogenic differentiation in mouse.